Alcarranza M. , Alarcón-De-la-Lastra C. , Recio Jiménez R. , Fernández I. , CASTEJÓN MARTÍNEZ, MARÍA LUISA, Villegas I.
No
J. Agric. Food Chem.
Article
Científica
1
1
6.2
01/01/2024
2-s2.0-85204547087
The present study aimed to investigate the effects of (R)-(-)-1-isothiocyanato-6-(methylsulfinyl)-hexane [(R)-6-HITC], the major isothiocyanate present in wasabi, in an ex vivo model of inflammation using lipopolysaccharide-stimulated murine peritoneal macrophages. (R)-6-HITC improved the immune response and mitigated oxidative stress, which involved suppression of reactive oxygen species, nitric oxide, and pro-inflammatory cytokines (IL-1ß, IL-6, IL-17, IL-18, and TNF-a) production and downregulation of pro-inflammatory enzymes such as inducible nitric oxide synthase, COX-2, and mPGES-1. In addition, (R)-6-HITC was able to activate the Nrf2/HO-1 axis while simultaneously inhibiting key signaling pathways, including JAK2/STAT3, mitogen-activated protein kinases, and canonical and noncanonical inflammasome pathways, orchestrating its potent immunomodulatory effects. Collectively, these findings demonstrate the potential of (R)-6-HITC as a promising nutraceutical for the management of immuno-inflammatory diseases and justify the need for further in vivo validation studies. © 2024 The Authors. Published by American Chemical Society.
Animals; Cyclooxygenase 2; Cytokines; Disease Models, Animal; Humans; Immunologic Factors; Immunomodulating Agents; Inflammation; Isothiocyanates; Lipopolysaccharides; Macrophages, Peritoneal; Male; Mice; Mice, Inbred C57BL; NF-E2-Related Factor 2; Nitric Oxide; Nitric Oxide Synthase Type II; Oxidative Stress; Plant Extracts; Reactive Oxygen Species; Signal Transduction; Wasabia; Macrophages; Mammals; Nitric oxide; Oxidative stress; Pathology; Reactive oxygen species; cyclooxygenase 2; cytokine; immunologic factor; immunomodulating agent; inducible nitric oxide synthase; isothiocyanic acid; lipopolysaccharide; nitric oxide; plant extract; reactive oxygen metabolite; transcription factor Nrf2; (R)-6-HITC; DAG-methodology; Effect mechanism; Eutrema japonicum; Ex-vivo; Immunomodulatory effects; Inflammation; Isothiocyanates; Murine peritoneal macrophages; Wasabi; animal; C57BL mouse; chemistry; disease model; drug effect; drug therapy; Eutrema; genetics; human; immunology; inflammation; m