de la Cruz-Ruiz, Patricia , Hernando-Rodriguez, Blanca , Perez-Jimenez, Mercedes M. , RODRIGUEZ PALERO, MARÍA JESÚS, Martinez-Bueno, Manuel D. , Pla, Antoni , Gatsi, Roxani , Artal-Sanz, Marta
No
Aging Cell
Article
Científica
1
1
11.005
2.751
01/05/2021
000646329500001
Mitochondrial prohibitins (PHB) are highly conserved proteins with a peculiar effect on lifespan. While PHB depletion shortens lifespan of wild-type animals, it enhances longevity of a plethora of metabolically compromised mutants, including target of rapamycin complex 2 (TORC2) mutants sgk-1 and rict-1. Here, we show that sgk-1 mutants have impaired mitochondrial homeostasis, lipogenesis and yolk formation, plausibly due to alterations in membrane lipid and sterol homeostasis. Remarkably, all these features are suppressed by PHB depletion. Our analysis shows the requirement of SRBP1/SBP-1 for the lifespan extension of sgk-1 mutants and the further extension conferred by PHB depletion. Moreover, although the mitochondrial unfolded protein response (UPRmt) and autophagy are induced in sgk-1 mutants and upon PHB depletion, they are dispensable for lifespan. However, the enhanced longevity caused by PHB depletion in sgk-1 mutants requires both, the UPRmt and autophagy, but not mitophagy. We hypothesize that UPRmt induction upon PHB depletion extends lifespan of sgk-1 mutants through autophagy and probably modulation of lipid metabolism.
autophagy; lipogenesis; mitochondria; prohibitin; SGK‐ 1; UPRmt