Osterwalder, Marco , Galli, Antonella , Rosen, Barry , Skarnes, William C. , Zeller, Rolf , LÓPEZ-RÍOS MORENO, JAVIER
Si
Nat. Methods
Article
Científica
20.721
8.798
01/11/2010
000283586600008
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.