Gomez Estevez, Paula , Cisneros, Jose Miguel , Lepe, Jose Antonio , MARTÍN GUTIÉRREZ, GUILLERMO, Ortiz de la Rosa, Jose Manuel
No
Sci Rep
Article
Científica
03/01/2026
001676676600003
The primary obstacle in detecting bloodstream infections is their often extremely low bacterial concentration. To accurately detect such infections, we developed a quantitative PCR assay, named LowLoad-qPCR, based on the combination of increased input DNA by random priming amplification method and the use of multiple probes sharing a single fluorophore. Here, we detail the key improvements over conventional qPCR and evaluate its performance using blood samples.
qPCR; Rapid diagnostic; Sensitivity; Blood infection; Bacteriemia