Camacho-Martinez, Pedro , MARTÍN GUTIÉRREZ, GUILLERMO, Penalva, German , Merino-Diaz, Laura , Antonio Lepe, Jose
No
Enferm. Infec. Microbiol. Clin.
Article
Científica
2.5
0.262
01/10/2022
000898444200006
Introduction: In the present manuscript we describe the planning carried out in our hospital to adapt our diagnostic capability to perform large numbers of SARS-CoV-2 RT-PCR. Methods: The analysis and prediction of workflow included the number of RT-PCR per week from the beginning of the pandemic, with a total of 31971 determinations. The planning phase was developed based on the different scenarios previously predicted. Results: According to the predictions obtained, an automated custom solution was chosen, based on the use of the OT-2 open-source liquid-handling robots (Opentrons), to design a reproducible workflow that achieved a production capacity of 5640 samples/day, with a time of response of four hours per procedure. Conclusions: The analysis and prediction of workflow, along with the use of the robotic platforms OT-2, provided a robust structure to deal with the high demand of determinations that this pandemic requires. (c) 2021 Sociedad Espa & ntilde;ola de Enfermedades Infecciosas y Microbiolog & iacute;a Cl & iacute;nica. Published by Elsevier Espa & ntilde;a, S.L.U. All rights reserved.
SARS-CoV-2; RT-PCR; Opentrons; Diagnostic capacity