CALDERON SANCHEZ, EVA MARIA, Dominguez-Rodriguez, A. , Rodriguez Moyano, M. , Ordonez, A. , Smani, T. , MEDIMOND
No
Proceedings Of The Xxviii European Section Meeting Of The International Society For Heart Research
Proceedings Paper
Científica
0
0
01/01/2008
000257541000004
Despite the considerable progress that has been made in the field of myocardial protection, high-risk subsets of patients continue to exhibit ischemia-reperfusion-related complications and represent a major public health problem. Recently, urocortin (Ucn) has been proposed as a new cardioprotector against chronic ischemia. We examined the effect of Ucn (10 nM) in isolated rat heart under acute ischemia/reperfusion (I/R). We compared the contractility (dP/dt) in heart submitted to ischemia (30 min) and reperfusion (2 h), and in hearts that were treated with Ucn alone or in presence of PKA, PKC, and K-ATP channels inhibitors. We found that Ucn induced a significant and potent recovery of heart contractility after I/R, which was independent of the activation of PKA and PKC pathway. However, hearts pre-treatment with glibenclamide to inhibit K-ATP channels prevented Ucn effect. Altogether indicates that Ucn induced heart protection against acute I/R injury involve K-ATP activation but not PKA and PKC pathway that was implicated in the later heart protection for example against apoptosis.