Camacho-Martínez, Pedro , MARTÍN GUTIÉRREZ, GUILLERMO, Peñalva, Germán , Merino-Díaz, Laura , Lepe, José Antonio
No
Enferm Infecc Microbiol Clin
Article
Científica
1.994
0.277
01/01/2021
INTRODUCTION: In the present manuscript we describe the planning carried out in our hospital to adapt our diagnostic capability to perform large numbers of SARS-CoV-2 RT-PCR. METHODS: The analysis and prediction of workflow included the number of RT-PCR per week from the beginning of the pandemic, with a total of 31971 determinations. The planning phase was developed based on the different scenarios previously predicted. RESULTS: According to the predictions obtained, an automated custom solution was chosen, based on the use of the OT-2 open-source liquid-handling robots (Opentrons), to design a reproducible workflow that achieved a production capacity of 5640 samples/day, with a time of response of four hours per procedure. CONCLUSIONS: The analysis and prediction of workflow, along with the use of the robotic platforms OT-2, provided a robust structure to deal with the high demand of determinations that this pandemic requires.
Capacidad diagnóstica; Diagnostic capacity; Opentrons; PCR-RT; RT-PCR; SARS-CoV-2