Perez-Gomez, Alberto , Gasca-Capote, Carmen , Vitalle, Joana , Ostos, Francisco J. , SERNA GALLEGO, ANA DEL ROSARIO, Trujillo-Rodriguez, Maria , Munoz-Muela, Esperanza , Giraldez-Perez, Teresa , Praena-Segovia, Julia , Navarro-Amuedo, Maria D. , Paniagua-Garcia, Maria , Garcia-Gutierrez, Manuel , Aguilar-Guisado, Manuela , Rivas-Jeremias, Inmaculada , Jimenez-Leon, Maria Reyes , Bachiller, Sara , Fernandez-Villar, Alberto , Perez-Gonzalez, Alexandre , Gutierrez-Valencia, Alicia , Benhnia, Mohammed Rafii-El-Idrissi , Weiskopf, Daniela , Sette, Alessandro , Lopez-Cortes, Luis F. , Poveda, Eva , Ruiz-Mateos, Ezequiel , Virgen Rocio del Hosp COVID-19 Wor , Virgen Rocio del Hosp COHVID-GS Wo
No
Clin. Transl. Med.
Article
Científica
1
1
10.6
1.706
01/04/2022
000781826800001
SARS-CoV-2 specific T-cell response has been associated with disease severity, immune memory and heterologous response to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality of SARS-CoV-2 specific T-cell response with antibody levels in these three scenarios is needed. In the present study, we found that, in acute infection, while mild disease was associated with high T-cell polyfunctionality biased to IL-2 production and inversely correlated with anti-S IgG levels, combinations only including IFN-gamma with the absence of perforin production predominated in severe disease. Seven months after infection, both non-hospitalised and previously hospitalised patients presented robust anti-S IgG levels and SARS-CoV-2 specific T-cell response. In addition, only previously hospitalised patients showed a T-cell exhaustion profile. Finally, combinations including IL-2 in response to S protein of endemic coronaviruses were the ones associated with SARS-CoV-2 S-specific T-cell response in pre-COVID-19 healthy donors' samples. These results could have implications for protective immunity against SARS-CoV-2 and recurrent COVID-19 and may help for the design of new prototypes and boosting vaccine strategies.
COVID-19; endemic coronaviruses; IL-2; nucleocapsid; polyfunctionality; SARS-CoV-2; Spike; T-cell response