Bermudez-Oria, Alejandra , Fernandez-Prior, Africa , CASTEJÓN MARTÍNEZ, MARÍA LUISA, Rodriguez-Gutierrez, Guillermo , Fernandez-Bolanos, Juan
No
Food Chem
Article
Científica
1
1
8.5
1.745
01/09/2023
000980091100001
The main by-product from olive oil extraction (alperujo) was extracted with hot water, citric acid, natural deep eutectic solvent (choline chloride: citric acid), and only choline chloride. The purified extracts were composed of macromolecular complexes constituting polyphenols associated with pectin. The extracts were structurally characterized by FT-IR and solid-NMR spectroscopy and an in vitro test revealed distinct antioxidant and antiproliferative activity, depending on the extracting agents. The choline chloride-extracted complex contained the highest amount of polyphenols among the examined agents, which exhibited a strong antioxidant activity and significant antiproliferative capacity. However, the complex extracted by hot water showed the highest antiproliferative capacity in vitro against the colon carcinoma Caco-2 cell line. In this finding, choline chloride could be used as a novel, green and promising alternative to the conventional extracting agent for the production of complexes that combine the antioxidant activity of phenolic compounds and the physiological effects of pectic polysaccharides.
Pectin-polyphenols complex; Choline chloride; Natural deep eutectic solvent; NMR and IR spectroscopy; Antioxidant activity; Caco-2 cell line