| Título |
Extract From the Leaves of Polypodium Leucotomos Stimulates DNA Repair Signals ?H2AX and P53 to Prolong the Keratinocyte DNA Repair Phase of the Cell Cycle |
| Autores |
Gandarillas, Alberto , Guerrero, Antonio , Delgado-Rubin, Arancha , Gonzalez, Salvador , RODRÍGUEZ LUNA, AZAHARA MARÍA, Juan, Lucia San |
| Publicación externa |
No |
| Medio |
J. Cosmet. Sci. |
| Alcance |
Article |
| Naturaleza |
Científica |
| Cuartil JCR |
4 |
| Cuartil SJR |
4 |
| Fecha de publicacion |
01/05/2023 |
| ISI |
001094244100003 |
| Abstract |
Polypodium leucotomos extract (PLE) has been extensively utilized by both topical and oral administration due to its beneficial protective or repair effects on various cell types of the skin. Now, we have investigated the direct function of PLE on the proliferative capacity and the cell cycle of freshly isolated keratinocytes from human skin. To note, PLE significantly induced the phosphorylation of histone H2AX, an early signal that triggers the DNA repair machinery upon genetic damage, both in exponentially proliferating cells and upon ultraviolet light irradiation. Interestingly, the induction of the signal was independent of actual DNA breaks, as monitored by comet assays. PLE also induced p53, the "guardian of the genome" that halts cells in the G2 phase of the cell cycle to allow DNA repair. As a result, epidermal multipotent stem cells grew more slowly but were more protected as they displayed a more powerful amplification after the PLE treatment. We discuss a model of action of PLE on the keratinocyte cycle that might be common to other natural substances used, or with a potential to be used, in skin cosmetics. |
| Miembros de la Universidad Loyola |
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