Título Genetic Tools to Study Cardiovascular Biology
Autores Garcia-Gonzalez, Irene , Muhleder, Severin , FERNÁNDEZ CHACÓN, MACARENA, Benedito, Rui
Publicación externa Si
Medio Frontiers in Physiology
Alcance Review
Naturaleza Científica
Cuartil JCR 1
Cuartil SJR 2
Impacto JCR 4.566
Impacto SJR 1.32
Fecha de publicacion 24/09/2020
ISI 000575863200001
DOI 10.3389/fphys.2020.01084
Abstract Progress in biomedical science is tightly associated with the improvement of methods and genetic tools to manipulate and analyze gene function in mice, the most widely used model organism in biomedical research. The joint effort of numerous individual laboratories and consortiums has contributed to the creation of a large genetic resource that enables scientists to image cells, probe signaling pathways activities, or modify a gene function in any desired cell type or time point, a la carte. However, as these tools significantly increase in number and become more sophisticated, it is more difficult to keep track of each tool\'s possibilities and understand their advantages and disadvantages. Knowing the best currently available genetic technology to answer a particular biological question is key to reach a higher standard in biomedical research. In this review, we list and discuss the main advantages and disadvantages of available mammalian genetic technology to analyze cardiovascular cell biology at higher cellular and molecular resolution. We start with the most simple and classical genetic approaches and end with the most advanced technology available to fluorescently label cells, conditionally target their genes, image their clonal expansion, and decode their lineages.
Palabras clave genetic tools; mosaics; cardiovascular; imaging; barcoding; transgenics; Cre; clonal analysis
Miembros de la Universidad Loyola

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