Lantada A.D. , ENDRINO ARMENTEROS, JOSÉ LUIS, Vaquero V.S. , Mosquera A. , Lafont P. , García-Ruiz J.P.
Si
Plasma Process. Polym.
Article
Científica
3.73
1.212
01/01/2012
000298842100013
2-s2.0-84855560476
In this study we investigate an alternative method for designing and obtaining novel scaffolds for analyzing the influence of geometries on tissue engineering processes. The method is based on the combination of conventional and accessible rapid prototyping technologies with plasma processing using a hydrogen-free diamond-like carbon (DLC) coating in order to improve cell-tissue interactions. The proposed method allows a precise control of scaffold structure and enables in vitro studies linked to cell growth and tissue formation because of the highly biocompatible DLC surface. Several designs with different hole sizes and surface topographies have been manufactured for subsequent in vitro study of human mesenchymal stem cell (hMSC) growth and aggregation, so as to validate our approach. A method for designing and manufacturing novel polymeric scaffolds for tissue engineering is presented. The process is based on the combination of conventional and accessible rapid prototyping technologies with surface functionalization using a highly biocompatible diamond-like carbon (DLC) coating to improve cell-tissue interactions. Results from in vivo trials and potential implications are discussed. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Alternative methods; Diamond-like carbon; Diamond-like carbon coatings; Engineering process; Hole size; Human mesenchymal stem cells; In-vitro; In-vivo; Polymeric scaffold; Precise control; Rapid prototyping technology; Scaffold structures; Surface Functionalization; thermosetting polymers; Tissue formation; Cell culture; Coatings; Diamonds; Hydrogen; Manufacture; Polymers; Rapid prototyping; Scaffolds; Stem cells; Thermosets; Tissue; Tissue engineering; Scaffolds (biology)