Title Functional evaluation and testing of a newly developed Teleost\'s Fish Otolith derived biocomposite coating for healthcare
Authors Montanez, Nerly D. , Carreno, Heider , Escobar, Patricia , Estupinan, Hugo A. , Pena, Dario Y. , Goel, Saurav , ENDRINO ARMENTEROS, JOSÉ LUIS
External publication Si
Means Sci. Rep.
Scope Article
Nature Científica
JCR Quartile 1
SJR Quartile 1
JCR Impact 4.38000
SJR Impact 1.24000
Web https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077889096&doi=10.1038%2fs41598-019-57128-w&partnerID=40&md5=4b5da0b45ed97d6eed2312532642ddf3
Publication date 14/01/2020
ISI 000551361900005
Scopus Id 2-s2.0-85077889096
DOI 10.1038/s41598-019-57128-w
Abstract Polymers such as polycaprolactone (PCL) possess biodegradability, biocompatibility and affinity with other organic media that makes them suitable for biomedical applications. In this work, a novel biocomposite coating was synthesised by mixing PCL with layers of calcium phosphate (hydroxyapatite, brushite and monetite) from a biomineral called otolith extracted from Teleost fish (Plagioscion Squamosissimus) and multiwalled carbon nanotubes in different concentrations (0.5, 1.0 and 1.5 g/L). The biocomposite coating was deposited on an osteosynthesis material Ti6Al4V by spin coating and various tests such as Fourier transformation infrared spectroscopy (FTIR), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), scratch tests, MTT reduction cytotoxicity, HOS cell bioactivity (human osteosarcoma) by alkaline phosphatase (ALP) and fluorescence microscopy were performed to comprehensively evaluate the newly developed biocoating. It was found that an increase in the concentration of carbon nanotube induced microstructural phase changes of calcium phosphate (CP) leading to the formation of brushite, monetite and hydroxyapatite. While we discovered that an increase in the concentration of carbon nanotube generally improves the adhesion of the coating with the substrate, a certain threshold exists such that the best deposition surfaces were obtained as PCL/CP/CNT 0.0 g/L and PCL/CP/CNT 0.5 g/L.
Keywords alkaline phosphatase; biocompatible coated material; calcium phosphate; carbon nanotube; polycaprolactone; polyester; titanium; titanium alloy (TiAl6V4); animal; cell adhesion; cell survival; chemistr
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