Imoesi, Peter I. , OLARTE SÁNCHEZ, CRISTIAN MANUEL, Heisler, Lora , McCaffery, Peter
No
STAR Protoc.
Article
Científica
20/03/2026
001682897200001
The brain, specifically the hypothalamus, is a key regulator of the homeostatic balance of numerous biomolecules in the body via its control of the neuroendo-crine system. Here, we present a protocol to study body vitamin A balance by in-jecting synthetic retinoids into the third ventricle of the rat brain. We also describe steps for the intraventricular application of viral vectors to the arcuate nucleus of a mouse brain to knock down vitamin A homeostatic genes. This approach is applicable to the study of vitamin A homeostasis.
For complete details on the use and execution of this protocol, please refer to Imoesi et al.