Título Development of a novel immunobiosensor method for the rapid detection of okadaic acid contamination in shellfish extracts
Autores MOREJÓN LLAMAS, NOEMÍ, Stewart, Linda , Fodey, Terry , Higgins, H. Cowan , Velasco, Maria Luisa R. , Botana, Luis M. , Elliott, Christopher T.
Publicación externa Si
Alcance Article
Naturaleza Científica
Cuartil JCR 1
Cuartil SJR 2
Impacto JCR 2.86700
Impacto SJR 1.08500
Fecha de publicacion 01/09/2007
ISI 249008200026
DOI 10.1007/s00216-007-1444-3
Abstract The mouse bioassay is the methodology that is most widely used to detect okadaic acid (OA) in shellfish samples. This is one of the best-known toxins, and it belongs to the family of marine biotoxins referred to as the diarrhetic shellfish poisons (DSP). Due to animal welfare concerns, alternative methods of toxin detection are being sought. A rapid and specific biosensor immunoassay method was developed and validated for the detection of OA. An optical sensor instrument based on the surface plasmon resonance (SPR) phenomenon was utilised. A polyclonal antibody to OA was raised against OA-bovine thyroglobulin conjugate and OA-N-hydroxy succinimide ester was immobilised onto an amine sensor chip surface. The assay parameters selected for the analysis of the samples were: antibody dilution, 1/750; ratio of antibody to standard, 1:1; volume of sample injected, 25 mu l min(-1); flow rate, 25 mu l min(-1). An assay action limit of 126 ng g(-1) was established by analysing of 20 shellfish samples spiked with OA at the critical concentration of 160 ng g(-1), which is the action limit established by the European Union (EU). At this concentration of OA, the assay delivered coefficient of variations (CVs) of < 10%. The chip surface developed was shown to be highly stable, allowing more than 50 analyses per channel. When the concentrations of OA determined with the biosensor method were compared with the values obtained by LC-MS in contaminated shellfish samples, the correlation between the two analytical methods was found to be highly satisfactory (r(2)=0.991).
Palabras clave biosensors; okadaic acid; diarrhetic shellfish poisons; mouse bioassay
Miembros de la Universidad Loyola

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