| Title | Distribution and characteristics of ßII tubulin-enriched microtubules in interphase cells |
|---|---|
| Authors | Armas-Portela, R , PARRALES BORRERO, MIGUEL ÁNGEL, Albar, JP , Martinez-A, C , Avila, J |
| External publication | No |
| Means | Exp. Cell Res. |
| Scope | Article |
| Nature | Científica |
| JCR Quartile | 1 |
| SJR Quartile | 1 |
| JCR Impact | 3.256 |
| SJR Impact | 2.163 |
| Publication date | 01/05/1999 |
| ISI | 000080175300004 |
| DOI | 10.1006/excr.1999.4426 |
| Abstract | We have used a polyclonal antibody (Ab196) that specifically recognizes the beta II tubulin isotype to examine the subcellular distribution and properties of microtubules enriched in this isotype. Antibody specificity was tested by a method that involves the analysis of its interaction with individual beta isotypes. Using photoimaging analysis, we observed beta II tubulin-enriched microtubules in the perinuclear region, as well as in the microtubules close to the periphery of interphase cells. The observed sorting of beta II-enriched microtubules together with the reported increased levels of beta II tubulin in taxol-resistant cells (IM. Haber et al., 1995, J. Biol. Chem. 270, 31269-31275) prompted us to study the behavior of microtubules enriched in this isotype after different depolymerizing treatments. After cold or nocodazol treatments, beta II-enriched microtubules anchored at the centrosome and at the cell periphery were observed. In addition, cold-resistant microtubules were marked mainly by the specific anti-beta II tubulin antibody but not by anti-acetylated alpha tubulin, suggesting the presence of different stable microtubule subsets enriched in particular tubulin isoforms. (C) 1999 Academic Press. |
| Keywords | beta II tubulin; microtubule cold-stability; microtubule depolymerizing drug resistance |
| Universidad Loyola members |