Title Merging a sensitive capillary electrophoresis-ultraviolet detection method with chemometric exploratory data analysis for the determination of phenolic acids and subsequent characterization of avocado fruit
Authors HURTADO FERNÁNDEZ, ELENA, Contreras-Gutierrez, Paulina K. , Cuadros-Rodriguez, Luis , Carrasco-Pancorbo, Alegria , Fernandez-Gutierrez, Alberto
External publication Si
Means Food Chem.
Scope Article
Nature Científica
JCR Quartile 1
SJR Quartile 1
JCR Impact 3.25900
SJR Impact 1.55400
Publication date 15/12/2013
ISI 000324848000029
DOI 10.1016/j.foodchem.2013.06.007
Abstract Herein we present the development of a powerful CE-UV method able to detect and quantify an important number of phenolic acids in 13 varieties of avocado fruits at 2 ripening stages. All the variables involved in CE separation were exhaustively optimized and the best results were obtained with a capillary of 50 mu m i.d. x 50 cm effective length, sodium tetraborate 40 mM at a pH of 9.4, 30 kV, 25 degrees C, 10 s of hydrodynamic injection (0.5 psi) and UV detection at 254 nm. This optimal methodology was fully validated and then applied to different avocado samples. The number of phenolic acids determined varied from 8 to 14 compounds; in general, they were in concentrations ranging from 0.13 ppm to 3.82 ppm, except p-coumaric, benzoic and protocatechuic acids, which were found at higher concentrations. Principal component analysis (PCA) was applied to highlight the differences between varieties and ripening degrees, looking for the most influential analytes. (c) 2013 Elsevier Ltd. All rights reserved.
Keywords Phenolic acids; Avocado; Persea americana; Capillary electrophoresis; Principal component analysis
Universidad Loyola members

Change your preferences Manage cookies