Title Metabolomic analysis of avocado fruits by GC-APCI-TOF MS: effects of ripening degrees and fruit varieties
Authors HURTADO FERNÁNDEZ, ELENA, Pacchiarotta, T. , Mayboroda, O. A. , Fernandez-Gutierrez, A. , Carrasco-Pancorbo, A.
External publication Si
Means Anal. Bioanal. Chem.
Scope Article
Nature Científica
JCR Quartile 1
SJR Quartile 1
JCR Impact 3.12500
SJR Impact 1.05900
Publication date 01/01/2015
ISI 000348207200019
DOI 10.1007/s00216-014-8283-9
Abstract In order to investigate avocado fruit ripening, nontargeted GC-APCI-TOF MS metabolic profiling analyses were carried out. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to explore the metabolic profiles from fruit samples of 13 varieties at two different ripening degrees. Mannoheptulose; pentadecylfuran; aspartic, malic, stearic, citric and pantothenic acids; mannitol; and beta-sitosterol were some of the metabolites found as more influential for the PLS-DA model. The similarities among genetically related samples (putative mutants of "Hass") and their metabolic differences from the rest of the varieties under study have also been evaluated. The achieved results reveal new insights into avocado fruit composition and metabolite changes, demonstrating therefore the value of metabolomics as a functional genomics tool in characterizing the mechanism of fruit ripening development, a key developmental stage in most economically important fruit crops.
Keywords Gas chromatography; Food metabolomics; Atmospheric pressure chemical ionization; Persea americana; Principal component analysis
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