Title Profiling LC-DAD-ESI-TOF MS Method for the Determination of Phenolic Metabolites from Avocado (Persea americana)
Authors HURTADO FERNÁNDEZ, ELENA, Carrasco-Pancorbo, Alegria , Fernandez-Gutierrez, Alberto
External publication Si
Means J Agric Food Chem
Scope Article
Nature Científica
JCR Quartile 1
SJR Quartile 1
JCR Impact 2.82300
SJR Impact 1.38900
Publication date 23/03/2011
ISI 000288401800018
DOI 10.1021/jf104276a
Abstract A powerful HPLC-DAD-ESI-TOF MS method was established for the efficient identification of the chemical constituents in the methanolic extracts of avocado (Persea americana). Separation and detection conditions were optimized by using a standard mix containing 39 compounds belonging to phenolic acids and different categories of flavonoids, analytes that could be potentially present in the avocado extracts. Optimum LC separation was achieved on a Zorbax Eclipse Plus C18 analytical column (4.6 x 150 mm, 1.8 mu m particle size) by gradient elution with water + acetic acid (0.5%) and acetonitrile as mobile phases, at a flow rate of 1.6 mL/min. The detection was carried out by ultraviolet-visible absorption and ESI-TOF MS. The developed method was applied to the study of 3 different varieties of avocado, and 17 compounds were unequivocally identified with standards. Moreover, around 25 analytes were tentatively identified by taking into account the accuracy and isotopic information provided by TOF MS.
Keywords Liquid chromatography; time-of-flight-mass spectrometry; phenolic compounds; avocado; Persea americana; secondary metabolites
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