Title Quantitative characterization of important metabolites of avocado fruit by gas chromatography coupled to different detectors (APCI-TOF MS and FID)
Authors HURTADO FERNÁNDEZ, ELENA, Pacchiarotta, Tiziana , Mayboroda, Oleg A. , Fernandez-Gutierrez, Alberto , Carrasco-Pancorbo, Alegria
External publication Si
Means Food Res Int
Scope Article
Nature Científica
JCR Quartile 1
SJR Quartile 1
JCR Impact 2.81800
SJR Impact 1.48700
Publication date 01/08/2014
ISI 000340015100095
DOI 10.1016/j.foodres.2014.04.038
Abstract Herewith the development of a GC methodology, where two different detectors (FID and APCI-TOF MS) were simultaneously used, to determine quantitative changes in the metabolic profile of Persea americana is presented. The metabolic evolution of 13 avocado varieties was checked quantifying analytes belonging to several chemical families (organic adds, phytohormones, vitamins, flavonoids and phenolic acids). The method was fully validated and the analytical parameters of both detectors were compared, showing both of them acceptable analytical features. GC-FID showed better results in terms of precision (repeatability and intermediate precision), but LODs and LOQs were significantly higher (oscillating from 24.8 to 800 mu g L-1 and from 82.7 mu g L-1 to 2666.7 mu g L-1, respectively) than for MS (LODs were found within the range of 0.1-207.8 mu g L-1 and LOQs of 0.5-692.5 mu g L-1). A total of 27 compounds were quantified by GC-APCI-MS, whilst GC-FID allowed the proper quantification of 7 analytes. The concentration of organic adds, flavonoids and vitamins tend, in general, to decrease with the ripening process, whereas phenolic adds such as ferulic or p-coumaric adds usually increase their concentration as the fruit ripens. To corroborate further on the metabolic changes associated with the avocado varieties and/or ripening, we used principal component analysis, identifying quinic and p-coumaric acids, epicatechin and quercetin as some of the most influential compounds explaining the classification of the samples under study. (C) 2014 Elsevier Ltd. All rights reserved.
Keywords Gas chromatography; Food metabolomics; Atmospheric pressure chemical ionization; Persea americana; Flame ionization detector; Principal component analysis
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